Many studies have been conducted in pursuit of health and safety. This study aims to suggest a preventive and
therapeutic plan to address dental caries by confirming the antibacterial effect of Cibotium barometz J. Smith
(Cibotium barometz J. S), a natural material, on Streptococcus mutans (S. mutans), a type of bacteria that can
cause dental carries, and to maximize the efficiency of antibacterial activity by applying low-temperature
plasma from electric currents and magnetic fields. Low-temperature plasma can be applied to living tissue
without thermal damage, and the composition of plasma is diverse, so the application field is increasing. Cibotium
barometz J. S extract was treated with S. mutans diluted to 1 × 105 CFUs/mL from low concentration (0.01
mg/mL) to high concentration (45 mg/mL) for 6 and 24 hours. CFUs (colony forming units) were counted for
the corresponding time, and lowtemperature plasma was used in combination to maximize antibacterial effect.
The higher the concentration of Cibotium barometz J. S extract, the higher the death rate was, and antibacterial
activity increased when combined with low-temperature plasma, compared to using Cibotium barometz J. S
extract alone. The application of low-temperature plasma improves the penetration of Cibotium barometz J. S
extract safely and effectively to suppress the occurrence of dental caries, and has good antibacterial effect.
Therefore, low-temperature plasma is considered excellent in promoting oral health.