The suppression of cytokine storm in severe coronavirus disease 2019 (COVID-19) patients can be treated with
monoclonal antibody therapy against CD3 for T cell receptor inhibition. An optimized liquid phase as a CD3
antibody-magnetic nanoparticle (Ab-MNP) conjugate can inhibit the overactivation of T cells. We aim to analyze
the distribution of Fe in the spleen after acute administration of silica-conjugated amine magnetite (Fe3O4)
nanoparticles (35 nm) delivered by intravenous injection. The Fe element distribution and concentration levels
in spleen tissue were analyzed using energy dispersive spectroscopy (EDS) and inductively coupled plasmamass
spectrometry (ICP-MS). The experimental result is a difference in the concentration of Fe elements,
which was 1.89×103 mg/kg in the spleen of a control mouse not administered with MNPs, whereas increases significantly
to 1.93×10³ mg/kg in that of a mouse administered with MNPs. Further, time kinetic analysis of biochemical
and immunological parameters is required to confirm its suitability in bio-administration.