Magnetic fluid, a new type of magnetic material, is a colloidal liquid constituted of nano-scale ferromagnetic particles suspended in carrier fluid. Magnetic fluid sealing is one of the most successful applications of magnetic fluid. As a new type of seal offering the advantages of no leakage, long life and high reliability, the magnetic fluid seal has been widely utilized under vacuum- and low-pressure-differential conditions. In practical applications, for improved pressure capacity, a multistage sealing structure is always used. However, in engineering applications, a uniform distribution of magnetic fluid under each tooth often cannot be achieved, which problem weakens the overall pressure capacity of the seals. In order to improve the pressure capacity of magnetic fluid seals and broaden their applications, the present study theoretically and experimentally analyzed the degree of non-uniform distribution of multistage magnetic fluid seals. A mathematical model reflecting the relationship between the pressure capacity and the distribution of magnetic fluid under a single tooth was constructed, and a formula showing the relationship between the volume of magnetic fluid and its contact width with the shaft was derived. Furthermore, the relationship of magnetic fluid volume to capacity was analyzed. Thereby, the causes of non-uniform distribution could be verified: injection of magnetic fluid; the assembly of magnetic fluid seals; the change of magnetic fluid silhouette under pressure loading; the magnetic fluid sealing mechanism of pressure transmission, and seal failure. In consideration of these causes, methods to improve the pressure capacity of magnetic fluid seals was devised (and is herein proposed).