To suppress the wall slip effect and improve the yield stress measurement precision of magnetorheological fluid, measurement disks with different grooves are first manufactured. Then, the influence of groove characteristics on the yield stress of magnetorheological fluid is investigated by the method of experiments. Finally, the optimization wall grooves of measurement disks are obtained, and the yield stress of a self-prepared magnetorheological fluid is measured. Results indicate that the groove type and groove width have a slight influence on the shear yield stress, whereas the measured yield stress increases with enhanced groove density, and the optimized groove depth is 0.3 mm. The measured shear yield stress of self-prepared MR fluid can be improved by 18 % according to the optimized grooved disks, and the maximum yield stress can reach up to 65 kPa as the magnetic flux density is 0.5 T.