Magnetic particles in a novel, wound–healing ointment were studied using Mössbauer spectroscopy and VSM to estimate the stability of the properties of the magnetic particles. The isomer shifts of Fe₃O₄(A) were found to be 0.49-0.56 mm/s relative to iron metal, this indicates that the iron ions inFe₃O₄(A) are Fe³⁺. On the other hand, the isomer shifts of Fe₃O₄(B) were found to be 0.91-1.13 mm/s relative to iron metal, this shows that the ion state of Fe₃O₄(B) is a mixed state of Fe²⁺ and Fe³⁺. It is noted that this composition, as well as that of the initial pure component in the form of a highly dispersed fraction (~10 Å), differs from the stoichiometric one. It was found that the area ratio of the Mössbauer subspectra of Fe₃O₄(A) / Fe₃O₄(B) taken at 87 and 181 K linearly increased in comparison to the initial pure magnetic particles, but the rate of increase of the area ratio at 181 K was about two times that at 87 K. From the magnetic hyperfine field, despite their small size, the particles exhibit no superparamagnetism.